1H, 15N and 13C resonance assignments of light organ-associated fatty acid-binding protein of Taiwanese fireflies

Fatty acid-binding proteins (FABPs) are a family of proteins that modulate the transfer of various fatty acids in the cytosol and constitute a significant portion in many energy-consuming cells. The ligand binding properties and specific functions of a particular type of FABP seem to be diverse and depend on the respective binding cavity as well as the cell type from which this protein is derived. Previously, a novel FABP (lcFABP; lc: Luciola cerata) was identified in the light organ of Taiwanese fireflies. The lcFABP was proved to possess fatty acids binding capabilities, especially for fatty acids of length C-14-C-18. However, the structural details are unknown, and the structure-function relationship has remained to be further investigated. In this study, we finished the H-1, N-15 and C-13 chemical shift assignments of N-15/C-13-enriched lcFABP by solution NMR spectroscopy. In addition, the secondary structure distribution was revealed based on the backbone N, H, C-alpha, H-alpha, C and side chain C-beta assignments. These results can provide the basis for further structural exploration of lcFABP.