Journal
ARCHIVES OF MICROBIOLOGY
Volume 199, Issue 1, Pages 29-35Publisher
SPRINGER
DOI: 10.1007/s00203-016-1271-z
Keywords
Cyanobacteria; Halotolerant; Photorespiration; Salt stress; Serine; Serine hydroxymethyltransferase
Categories
Funding
- Ministry of Education, Science and Culture of Japan
- International Center for Green Biotechnology of Meijo University
- Grants-in-Aid for Scientific Research [15K18743] Funding Source: KAKEN
Ask authors/readers for more resources
Serine hydroxymethyltransferase (SHMT) catalyzes the conversion of serine to glycine and provides activated one-carbon units required for synthesis of nucleic acids, proteins and numerous biological compounds. SHMT is involved in photorespiratory pathway of oxygenic photosynthetic organisms. Accumulating evidence revealed that SHMT plays vital role for abiotic stresses such as low CO2 and high salinity in plants, but its role in cyanobacteria remains to be clarified. In this study, we examined to overexpress the SHMT from halotolerant cyanobacterium Aphanothece halophytica in freshwater cyanobacterium, Synechococcus elongatus PCC7942. The transformed cells did not show an obvious phenotype under non-stress condition, but exhibited more tolerance to salinity than the control cells harboring vector only under high salinity. Elevated levels of enzymes in phosphorylated serine biosynthetic pathway and photorespiration pathway were observed in the transformed cells. Glycine level was also increased in the transformed cells. Physiological roles of SHMT for salt tolerance were discussed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available