Journal
ARCHIVES OF MICROBIOLOGY
Volume 198, Issue 9, Pages 885-892Publisher
SPRINGER
DOI: 10.1007/s00203-016-1248-y
Keywords
Mucoid conversion; Pseudomonas aeruginosa; AlgW; Truncated type IV pilin
Categories
Funding
- Chinese Academy of Science [Y15103-1-401]
- One-Hundred Scholar Award
- Guizhou Provincial People's Hospital Doctor Fund Project [GZSYBS[2015]04]
- National Aeronautics and Space Administration West Virginia Space Grant Consortium (NASA WVSGC)
- Cystic Fibrosis Foundation [CFF-YU11G0]
- NIH [P20GM103434]
- NASA WVSGC Graduate Research Fellowship
Ask authors/readers for more resources
For alginate production in Pseudomonas aeruginosa, the intramembrane protease AlgW must be activated to cleave the periplasmic domain of anti-sigma factor MucA for release of the sequestered ECF sigma factor AlgU. Previously, we reported that three tandem point mutations in the pilA gene, resulting in a truncated type IV pilin termed PilA(108) with a C-terminal motif of phenylalanine-threonine-phenylalanine (FTF), induced mucoidy in strain PAO579. In this study, we purified PilA(108) protein and synthesized a peptide 'SGAGDITFTF' corresponding to C-terminus of PilA108 and found they both caused the degradation of MucA by AlgW. Interestingly, AlgW could also cleave PilA(108) between alanine(62) and glycine(63) residues. Overexpression of the recombinant FTF motifbearing MucE protein, originally a small periplasmic
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available