Journal
BIOMICROFLUIDICS
Volume 9, Issue 1, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.4905840
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Funding
- National Institutes of Health [R01 EB006124]
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We report the successful fabrication and testing of 3D printed microfluidic devices with integrated membrane-based valves. Fabrication is performed with a low-cost commercially available stereolithographic 3D printer. Horizontal microfluidic channels with designed rectangular cross sectional dimensions as small as 350 mu m wide and 250 mu m tall are printed with 100% yield, as are cylindrical vertical microfluidic channels with 350 mu m designed (210 mu m actual) diameters. Based on our previous work [Rogers et al., Anal. Chem. 83, 6418 (2011)], we use a custom resin formulation tailored for low non-specific protein adsorption. Valves are fabricated with a membrane consisting of a single build layer. The fluid pressure required to open a closed valve is the same as the control pressure holding the valve closed. 3D printed valves are successfully demonstrated for up to 800 actuations. (c) 2015 AIP Publishing LLC.
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