Journal
WATER
Volume 11, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/w11051093
Keywords
horseradish peroxidase; 2-methyl-6-ethylaniline; removal efficiency; response surface methodology
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Funding
- National Key Technology RD Program [2015BAC05B05]
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For optimizing the reaction conditions of 2-methyl-6-ethylaniline (MEA) degradation catalyzed by horseradish peroxidase (HRP), a response surface methodology with three factors and three levels was used in this research to establish a regression model, a ternary quadratic polynomial, in order to analyze temperature, H2O2 concentration and pH effects on MEA removal efficiency. The results showed that the regression model was significant (p < 0.0001), fitted well with experimental data and had a high degree of reliability and accuracy, and the data were reasonable with low errors. By analyzing interactions and solving the regression model, the maximum MEA removal efficiency was 97.90%, and the optimal conditions were defined as follows: pH 5.02, H2O2 concentration 13.41mM, and temperature 30.95 degrees C. Under the optimal conditions, the average MEA removal efficiency obtained from the experiments was 97.56%. This research can provide reference for the treatment of actual acetochlor industrial wastewater.
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