Journal
ARCHIVES OF MEDICAL RESEARCH
Volume 47, Issue 6, Pages 411-418Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.arcmed.2016.09.005
Keywords
COPB2; Lentivirus; Prostate cancer; Proliferation; Inununohistochemistry
Categories
Funding
- National Natural Science Foundation of China [81372316]
- Science and Technology Development Fund in Wuxi [CSE31N1605]
- Key Laboratory of Malignant Tumor Molecular Mechanism and Translational Medicine of Guarngzhou Bureau of Science and Information Technology [[2013]163]
- Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangdong Higher Education Institutes [K1809001]
- Hospital Management Center in Wuxi [YGZXL1318]
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Background and Aims. Transport of membranes and proteins in eukaryotic cells is mediated by vesicular carriers. Coatomer complex I (COPI)-coated vesicles are involved in the transport between endoplasmic reticulum (ER) and Golgi complex. Several studies indicated that some subunits of COPI were correlated with the cell proliferation of malignant tumors. The present study focused on the function of coatomer protein complex subunit beta 2 (COPB2), one of seven proteins in COPI, in prostate cancer (PCa). Methods. COPB2 gene expression was first analyzed by immunohistochemistry (IHC) in 15 paired PCa and carcinoma adjacent normal tissue from patients. To investigate the role of COPB2 in PCa, we used lentivirus-mediated small interfering RNA (siRNA) to knockdown COPB2 expression in human PCa cell line PC-3 and assessed it by RT-qPCR. Cellomics ArrayScan VTI imaging and colony formation were conducted to evaluate cell proliferation. Cell cycle phase arrest and apoptosis were assayed by flow cytometry. Results. COPB2 gene was upregulated in the PCa tissue. Cell proliferation was significantly inhibited in COPB2-silenced PC-3 cells using both Cellomics ArrayScan VTI imaging and colony formation assays. S-phase cell counts were significantly decreased; Gl- and G2-phase cell counts were significantly increased in COPB2-siRNA group than the control group. Apoptosis was significantly increased in COPB2-siRNA cells. Conclusions. COPB2 significantly promoted PC-3 cell proliferation and colony formation through the cell cycle and apoptosis pathway. Moreover, COPB2 showed a clinical correlation and may serve as a biomarker for the detection for PCa. (C) 2016 IMSS. Published by Elsevier Inc.
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