Journal
CELL REPORTS
Volume 28, Issue 4, Pages 896-+Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2019.06.062
Keywords
-
Categories
Funding
- National Agency for the Promotion of Science and Technology (ANPCyT-FONCYT) [PICT 2015-2573, 2016-1740, 2016-1470, 2015-1859]
- National Scientific and Technical Research Council (CONICET) [PIP 2015-0567]
- Heart and Stroke Foundation of Canada (HSFC)
- CONICET
- ANPCyT-FONCYT
- Beverley Phillips Rising Star Postdoctoral Fellowship
- University of Calgary, Cumming School of Medicine Postdoctoral Scholar Program
- Canada Research Chairs program
Ask authors/readers for more resources
We investigated the contribution of human platelets to macrophage effector properties in the presence of lipopolysaccharide (LPS), as well as the beneficial effects and time frame for platelet transfusion in septic animals. Our results show that platelets sequester both pro-(TNF-alpha/IL-6) and anti-(IL-10) inflammatory cytokines released by monocytes. Low LPS concentrations (0.01 ng/mL) induced M2 macrophage polarization by decreasing CD64 and augmenting CD206 and CD163 expression; yet, the presence of platelets skewed monocytes toward type 1 macrophage (M1) phenotype in a cell-contact-dependent manner by the glycoprotein lb (GP1b)-CD11b axis. Accordingly, platelet-licensed macrophages showed increased TNF-alpha levels, bacterial phagocytic activity, and a reduced healing capability. Platelet transfusion increased inducible nitric oxide synthase (iNOS)(+) macrophages, improving bacterial clearance and survival rates in septic mice up to 6 h post-infection, an effect that was abolished by CD11b and GPIb blockade. Our results demonstrate that platelets orchestrate macrophage effector responses, improving the clinical outcome of sepsis in a narrow but relevant time frame.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available