Journal
ADVANCED HEALTHCARE MATERIALS
Volume 8, Issue 15, Pages -Publisher
WILEY
DOI: 10.1002/adhm.201900425
Keywords
3D electrical stimulation; conductive biogels; human neural tissue; printed conducting polymer electrodes; stem cells
Funding
- Australian Research Council [FL110100196] Funding Source: Australian Research Council
- ARC Laureate Fellowship [FL110100196] Funding Source: Medline
- Australian Research Council [CE140100012] Funding Source: Medline
- Australian Research Council (ARC) Centre of Excellence Scheme [CE140100012] Funding Source: Medline
Ask authors/readers for more resources
Electricity is important in the physiology and development of human tissues such as embryonic and fetal development, and tissue regeneration for wound healing. Accordingly, electrical stimulation (ES) is increasingly being applied to influence cell behavior and function for a biomimetic approach to in vitro cell culture and tissue engineering. Here, the application of conductive polymer (CP) poly(3,4-ethylenedioxythiophene)-polystyrenesulfonate (PEDOT:PSS) pillars is described, direct-write printed in an array format, for 3D ES of maturing neural tissues that are derived from human neural stem cells (NSCs). NSCs are initially encapsulated within a conductive polysaccharide-based biogel interfaced with the CP pillar microelectrode arrays (MEAs), followed by differentiation in situ to neurons and supporting neuroglia during stimulation. Electrochemical properties of the pillar electrodes and the biogel support their electrical performance. Remarkably, stimulated constructs are characterized by widespread tracts of high-density mature neurons and enhanced maturation of functional neural networks. Formation of tissues using the 3D MEAs substantiates the platform for advanced clinically relevant neural tissue induction, with the system likely amendable to diverse cell types to create other neural and non-neural tissues. The platform may be useful for both research and translation, including modeling tissue development, function and dysfunction, electroceuticals, drug screening, and regenerative medicine.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available