Journal
SCIENTIFIC REPORTS
Volume 9, Issue -, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41598-019-42332-5
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Funding
- National Genetically Modified Organisms Breeding Major Projects [2016ZX08004-002, 2016ZX08004-005]
- Modern Agro-industry Technology Research Platform, Soybean Agro-industry Technology Research Platform [CARS-04]
- Science and technology innovation project of Chinese Academy of Agricultural Sciences, Molecular Mechanism Analysis and Germplasm Innovation of Soybean Stress Resistance to Tolerance and Nutrient Efficient Utilization [CAAS-ASTIP-2016-OCRI]
- Study on Molecular Assisted Breeding System for Drought Resistance and Lodging Resistance in Soybean [2012BB043]
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As major environment factors, drought or high salinity affect crop growth, development and yield. Transgenic approach is an effective way to improve abiotic stress tolerance of crops. In this study, we comparatively analyzed gene structures, genome location, and the evolution of syntaxin proteins containing late embryogenesis abundant (LEA2) domain. GmSYP24 was identified as a dehydration-responsive gene. Our study showed that the GmSYP24 protein was located on the cell membrane. The overexpression of GmSYP24 (GmSYP24ox) in soybean and heteroexpression of GmSYP24 (GmSYP24hx) in Arabidopsis exhibited insensitivity to osmotic/drought and high salinity. However, wild type soybean, Arabidopsis, and the mutant of GmSYP24 homologous gene of Arabidopsis were sensitive to the stresses. Under the abiotic stresses, transgenic soybean plants had greater water content and higher activities of POD, SOD compared with non-transgenic controls. And the leaf stomatal density and opening were reduced in transgenic Arabidopsis. The sensitivity to ABA was decreased during seed germination of GmSYP24ox and GmSYP24hx. GmSYP24hx induced up-regulation of ABA-responsive genes. GmSYP24ox alters the expression of some aquaporins under osmotic/drought, salt, or ABA treatment. These results demonstrated that GmSYP24 played an important role in osmotic/drought or salt tolerance in ABA signal pathway.
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