Journal
SCIENTIFIC REPORTS
Volume 9, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-019-41980-x
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Funding
- CINES (DARI project) [A0020707530]
- PRACE [2017174234]
- ANR (Agence nationale de la recherche) [ANR 10-BINF-0003, ANR-17-CE11-0011]
- CHEMISYST labex
- Agence Nationale de la Recherche (ANR) [ANR-17-CE11-0011, ANR-10-BINF-0003] Funding Source: Agence Nationale de la Recherche (ANR)
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Through their coupling to G proteins, G Protein-Coupled Receptors (GPCRs) trigger cellular responses to various signals. Some recent experiments have interestingly demonstrated that the G protein can also act on the receptor by favoring a closed conformation of its orthosteric site, even in the absence of a bound agonist. In this work, we explored such an allosteric modulation by performing extensive molecular dynamics simulations on the adenosine A2 receptor (A2AR) coupled to the Mini-Gs protein. In the presence of the Mini-Gs, we confirmed a restriction of the receptor's agonist binding site that can be explained by a modulation of the intrinsic network of contacts of the receptor. Of interest, we observed similar effects with the C-terminal helix of the Mini-Gs, showing that the observed effect on the binding pocket results from direct local contacts with the bound protein partner that cause a rewiring of the whole receptor's interaction network.
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