Journal
NATURE COMMUNICATIONS
Volume 10, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-019-09722-9
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Funding
- ERC starting grant COMPUCELL
- French Infrastructure for Integrated Structural Biology (FRISBI) [ANR-10-INSB-05-01]
- BBSRC/EPSRC [BB/M017702/1]
- ANR [ANR-15-CE1-0008]
- DGA (French Ministry of Defense)
- INRA
- IDEX Paris-Saclay
- Ecole Polytechnique
- BBSRC [BB/M017702/1] Funding Source: UKRI
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Cell-free transcription-translation systems have great potential for biosensing, yet the range of detectable chemicals is limited. Here we provide a workflow to expand the range of molecules detectable by cell-free biosensors through combining synthetic metabolic cascades with transcription factor-based networks. These hybrid cell-free biosensors have a fast response time, strong signal response, and a high dynamic range. In addition, they are capable of functioning in a variety of complex media, including commercial beverages and human urine, in which they can be used to detect clinically relevant concentrations of small molecules. This work provides a foundation to engineer modular cell-free biosensors tailored for many applications.
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