Journal
EPIGENOMICS
Volume 11, Issue 11, Pages 1335-1353Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/epi-2019-0121
Keywords
beta-catenin; circRNAs; epithelial to mesenchymal transition; glioblastoma; miR-326; Wnt7B
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Funding
- Natural Science Foundation of Guangdong Province [2015A030313465]
- Medical Research Fund of Guangdong Province [C2015037]
- Science and Technology Project of Guangdong Province [2014A020212337]
- Scientific and Technological Planning Project of Guangzhou City [1201420240]
- Science and Technology Planning Project of Guangzhou Education Bureau [1201630115]
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Aim: To study the role of circRNA (circ_0001730) in glioblastoma. Materials & methods: The interaction between circ 0001730 and miR-326 was confirmed by FISH, RNA pull down, RNA-binding protein immunoprecipitation and luciferase reporter assays. Cell proliferation and growth were determined by MTT, EdU and colony formation assays. Cell migration was assessed by the Boyden assay. Results: The levels of circ_0001730 were elevated in glioblastoma cell lines and tissues. circ_0001730 downregulation suppressed migration and proliferation in glioblastoma cells. SP1 bounds to the promoter of circ 0001730 host gene EPHB4 thereby increasing the expression of circ_0001730. circ_0001730 activated the Wnt/beta-catenin pathway via the miR-326/Wnt7B axis. Conclusion: circ_000173 promoted growth and invasion in glioblastoma cells via the miR-326/Wnt7B axis.
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