Journal
EXPERIMENTAL AND THERAPEUTIC MEDICINE
Volume 18, Issue 4, Pages 2401-2412Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/etm.2019.7753
Keywords
aptamer; RNA interference; breast cancer; aptamer-siRNA chimera; targeted cancer therapy; human epidermal growth factor receptor-3
Categories
Funding
- Florindon Foundation
- German Research foundation, DFG (Graduiertenkolleg 'Biointerface' 1035)
- Sixth Research Framework Programme of the European Union, Project RIGHT [LSHB-CT-2004-005276]
Ask authors/readers for more resources
Breast cancer is the most common cancer in women worldwide. Despite recent developments in breast cancer detection and treatment, 1.38 million women each year are still affected. Breast cancer heterogeneity at the population and single-cell level, complexity and developing different metastases are setting several challenges to develop efficient breast cancer therapies. RNA interference (RNAi) represents an opportunity to silence gene expression and inhibit specific pathways in cancer cells. In order to reap the full advantages of RNAi-based therapy, different pathways that sustain cancer cells growth have been targeted using specific siRNAs. The present study investigated the ability of a set of cytotoxic siRNAs to inhibit growth of breast cancer cells. These siRNAs are targeting eukaryotic elongation factor 2 (EEF2), polo-like kinase 1 (PLK1), G protein-coupled receptor kinase 4 (GRK4) and sphingosine kinase interacting protein (SKIP5). To facilitate their targeted delivery, the human epidermal growth factor receptor-3 (HER3)-specific aptamer A30 was used. The in vitro results described in this work indicate that combining the highly specific HER3 aptamer with cytotoxic siRNAs targeting (EEF2, PLK1, GRK4 and SKIP5) can inhibit its activity and ultimately suppress proliferation of HER3 positive breast cancer cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available