Journal
VIRUSES-BASEL
Volume 11, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/v11060544
Keywords
porcine circovirus type 2 (PCV2); 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR); adenosine 5 '-monophosphate (AMP)-activated protein kinase (AMPK); protein phosphatase 2 (PP2A); interaction
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Funding
- National Key Research and Development Program of China [2017YFD0500103]
- National Natural Science Foundation of China [31772747]
- Science and Technology Research Program during the 13th Five-Year Plan Period of the Jilin Educational Committee [JJKH20190172KJ]
- Program for JLU Science and Technology Innovative Research Team (JLUSTIRT) [2017TD-28]
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Porcine circovirus type 2 (PCV2) is the etiological agent of porcine circovirus diseases and porcine circovirus-associated diseases (PCVDs/PCVADs). However, the pathogenesis of PCV2 is not fully understood. We previously found that 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) is negatively associated with PCV2 infection in vitro and in vivo. HMGCR inhibits the early stages of PCV2 infection, while PCV2 infection induces the phosphorylation of HMGCR to inactivate the protein. In this study, we investigated the possibility that adenosine 5 ' -monophosphate (AMP)-activated protein kinase (AMPK), and protein phosphatase 2 (PP2A) participate in HMGCR-mediated inhibition of PCV2 infection and the interaction of porcine HMGCR with PCV2 proteins. The results showed that AMPK activity fluctuated in cells during the early stage of PCV2 infection, while PP2A had little effect on PCV2 infection and HMGCR activity. Furthermore, PCV2 infection may enhance or maintain the level of phosphorylated HMGCR by directly interacting with the protein in PK-15 cells. These findings may provide a better understanding of PCV2 pathogenesis, and HMGCR may be a novel PCV2 antiviral target.
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