Journal
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
Volume 74, Issue 9-10, Pages 265-273Publisher
WALTER DE GRUYTER GMBH
DOI: 10.1515/znc-2018-0114
Keywords
anti-obesity; homoisoflavonoid; Portulaca oleracea; 3T3-L1 cell
Funding
- National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2017R1A2B4009588]
- National Research Foundation of Korea (NRF)
- Center for Women In Science, Engineering and Technology (WISET) - Ministry of Science and ICT (MSIT) under the Program for Returners into RD [WISET-2018-792]
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This study was performed to isolate antiobesity components from the crude extract of Portulaca oleracea. The crude extract was partitioned into n-hexane, 85% aqueous methanol, n-butanol, and water fractions. Their effects on adipogenic differentiation were evaluated in 3T3-L1 cells. Among the solvent fractions from P. olearacea, the 85% aq. MeOH effectively reduced the levels of lipid accumulation. Further purification of 85% aq. MeOH led to the isolation of the known homoisoflavonoids 1-4, as the active substances. The administration of homoisoflavonoids to adipocyte cells decreased the lipid accumulation and glucose consumption and increased the release of glycerol into culture medium. In particular, homoisoflavonoid 3 effectively down-regulated the adipogenic transcription genes such as peroxisome proliferator activated receptor-gamma (PPAR gamma) and CCAAT/enhancer-binding proteins (C/EBP alpha), and adipogenic target genes such as fatty acid binding protein 4 (FABP4), fatty acid transport protein 1 (FATP1), and acyl-CoA synthase 1 (ACS1).
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