Cell Proliferation in the Isolated Brains of Adult Zebrafish Incubated in Artificial Cerebrospinal Fluid

The high rate of cell proliferation in the zebrafish brain allows one to perform reliable studies on cellular molecules associated with cell proliferation. In this study, we show that 5-bromo-2 '-deoxyuridine (BrdU)-labeled cells were detected in the isolated brains of adult zebrafish that were incubated in oxygenated artificial cerebrospinal fluid (aCSF) in vitro. The number of BrdU-labeled cells shown in the in vitro preparation was close to that in the in vivo study and was modified by treatment with various pharmacological agents, such as valproic acid. In parallel to cell proliferation, protein expression and neural activity could also be measured in the isolated brains maintained in aCSF.