Journal
VIBRATIONAL SPECTROSCOPY
Volume 103, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.vibspec.2019.102932
Keywords
Alginate oligomer; Cystic fibrosis; FTIR; Mucin; Sputum
Categories
Funding
- European Union via the Eurostars (TM) Programme
- European Social Fund
- Research Council of Norway
- Cystic Fibrosis Foundation US
- AlgiPharma AS
- Health Care Research Wales
- Welsh Government's Health Technology and Telehealth Fund
Ask authors/readers for more resources
Cystic fibrosis (CF) is an autosomal recessive, life-limiting condition characterized by progressive lung disease, which is a major cause of morbidity and mortality for these patients. The inhalation therapy, OligoG CF-5/20, is a low molecular weight (mean Mn 3200 g/mol) alginate oligomer, with a high guluronic acid content (>85%). The ability of OligoG CF-5/20 to enhance the activity of antimicrobial/antibiotic therapies, modify the rheological properties of CF sputum and interact with mucin, has previously been shown. To further characterize the physicochemical interactions of OligoG CF-5/20 with CF sputum, Fourier-transform infrared (FTIR) spectroscopy was used to analyze ex vivo sputum samples from adolescent CF patients (n = 13) following treatment with 0.2% OligoG CF-5/20. FTIR analysis confirmed the interaction of OligoG CF-5/20 with mucin glycans in CF sputum, which showed a shift in wavenumber from 1078 cm(-1) to 1070 cm(-1) and subsequent loss of the 1053 cm(-1) peak in the OligoG CF-5/20 treated samples. OligoG CF-5/20 interaction with key terminal moieties in mucin were also evident, with a significant change in sulphation at wavenumber 1116 cm(-1), suggesting a link with sulphated Lewis x antigen. There were also significant shifts at wavenumber 1637 cm(-1) indicative of beta-sheet conformational changes in the mucin peptide caused by action of OligoG. The alterations in charge of glycan and mucin structures support previous observations wherein OligoG CF-5/20 modifies the viscoelastic properties of CF sputum. These findings suggest a possible mechanism of action for the rheological changes observed with this novel therapy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available