Journal
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
Volume 117, Issue -, Pages 84-100Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2019.07.018
Keywords
Microfluidic; CTCs; Cell separation; Cell analysis; Biomedical applications
Categories
Funding
- National Key R&D Program of China [2017YFA0205901]
- National Natural Science Foundation of China [21535001, 81730051, 21761142006]
- Chinese Academy of Sciences [QYZDJ-SSW-SLH039, 121D11KYSB20170026]
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Metastasis is the leading cause of epithelial cancer-associated mortality. The shedding of tumor cells into the circulatory system is a key step for metastasis. Circulating tumor cells (CTCs) are reliable biomarkers for cancer diagnostics and treatment. To obtain clinical information, accurate approaches for separating and analyzing CTCs are necessary. Microfluidic technologies have demonstrated their extraordinary abilities for separating and analyzing CTCs. In this paper, we present a comprehensive review of microfluidics-based approaches for CTCs separation and analysis. We first present the general methods for CTCs separation using the microfluidic chips, including physical properties-based approaches for CTCs enrichment and affinity-based approaches for CTCs capture, and compare the pros and cons of different separation technologies. Then we introduce microfluidics-based CTCs analysis on cellular and molecular levels and the biomedical applications of CTCs analysis. Finally, we summarize the development of microfluidics-based CTCs separation and analysis, and make a prospective of future research. (C) 2019 Elsevier B.V. All rights reserved.
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