Changes in sperm parameters of sex-reversed female mandarin fish Siniperca chuatsi during cryopreservation process

This works studies the physiological characteristics (sperm motility characteristics) and biochemical characteristics (DNA damage, enzymes activities and fertilization ability) of fresh and freeze-thawed semen of sex-reversed female mandarin fish Siniperca chuatsi (n = 30) obtained with the application of 17 alpha-methyl testosterone for sex reversal. The fresh sperm motility and fertilization rate of sex-reversed females were about 83% and 70% respectively which had no significant difference with normal males (p> 0.05). Except for the value of DNA damage, other values of sperm motility, related enzymes activities and fertilization rate of sex-reversed female sperm declined after a process of cryopreservation (p < 0.05). But the frozen sperm can still get nearly 60% of the fertilization rate. This study identified the physiological and biochemical characteristics of the fresh and cryopreserved sperm from sex-reversed female mandarin fish, and the sex-reversed female spermatozoa can be used for actual production. (C) 2019 Elsevier Inc. All rights reserved.