Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 288, Issue -, Pages 38-43Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.02.076
Keywords
Polymer dots; alpha-L-Fucosidase; Inner filter effect; Fluorescent sensor; Human serum
Funding
- Fundamental Research Funds for the Central Universities, China [2662018JC011, 2014QC007]
- National Nature Science Foundation of China [21402056]
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alpha-L-Fucosidase (AFu) is an important biomarker for early diagnosis of hepatocellular carcinoma and fucosidosis. In this study, a novel, sensitive and selective polymer dots (PDs) fluorescent sensing strategy based on inner filter effect was firstly developed for AFu activity determination. The PDs with a high quantum yield of 53.6% were synthesized by a mild one-pot method. The detection mechanism of this strategy was based on inner filter effect between PDs and p-nitrophenol (PNP) which was the hydrolyzate of 4-nitrophenyl-alpha-L-fucopyranoside (PNPF) catalyzed by AFu. The absorption of PNP overlapped the fluorescence excitation spectrum of the PDs, which resulted in a fluorescence quenching or weakening of PDs. The sensing system showed a good linear relationship within 0.01-0.9 U L-1 and provided a low AFu detection limit of 0.001 U L-1 (S/N = 3). This PDs sensor were successfully applied for the determination of AFu in human serum samples.
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