Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 669, Issue -, Pages 621-630Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2019.02.440
Keywords
Pollution adaptation; POPs toxicology; Comparative transcriptomics; Gene family expansion; Aryl-hydrocarbon receptor; CYP3A
Categories
Funding
- National Natural Science Foundation of China [21525730, 21527901, 21836004]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB14030401, XDB14030402]
- Boston University Superfund Research Program (NIH) [P42ES007381]
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The freshwater climbing perch (Anabas testudineus) can tolerate water environments contaminated with persistent organic pollutants (POPs). The mechanisms underlying this tolerance are unknown. We used de novo transcriptomic analysis to investigate the defensive mechanisms of A. testudineus against POPs based on its genetic features and biological responses to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure. Our results revealed a specific expansion of cytochrome P450 (CYP) 3A subfamily, which may be involved in the elimination of certain POPs. In xenobiotic responses, the aryl-hydrocarbon receptor (AhR) pathway represents a critical signaling mechanism, and we characterized four AhR and two AhR nuclear translocator homologs and one AhR repressor (AhRR) gene in A. testudineus. TCDD-induced AhRR and CYP1A mRNA upregulation suggests that negative-feedback regulation of AhR signaling through AhRR helps avoid excessive xenobiotic responses. Furthermore, liver and gill transcriptomic profiles were markedly altered after TCDD exposure, with some of the altered genes being related to common defensive responses reported in other species. Based on the newly identified TCDD-altered genes, several A. testudineus-specific responses are proposed, such as enhanced fatty acid beta-oxidation. The genetic features of CYP3A subfamily and AhR pathway and the TCDD-induced defensive biological processes elucidated here enhance our understanding of A. testudineus defensive responses against POPs. (C) 2019 Elsevier B.V. All rights reserved.
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