Journal
SCIENCE
Volume 365, Issue 6454, Pages 699-+Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aav6416
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Funding
- HHMI
- Simons Collaboration on the Global Brain research awards [325171, 542943SPI]
- IARPA MICRONS [D16PC00003]
- NIH [R01EB22913]
- Taiwan Ministry of Science and Technology [MOST106-2628-B-010-004, MOST105-2628-B-010-005, MOST106-2320-B-010-012]
- Taiwan National Health Research Institute [NHRI-ex-107-10509NC]
- Allen Institute for Brain Science
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Genetically encoded voltage indicators (GEVIs) enable monitoring of neuronal activity at high spatial and temporal resolution. However, the utility of existing GEVIs has been limited by the brightness and photostability of fluorescent proteins and rhodopsins. We engineered a GEVI, called Voltron, that uses bright and photostable synthetic dyes instead of protein-based fluorophores, thereby extending the number of neurons imaged simultaneously in vivo by a factor of 10 and enabling imaging for significantly longer durations relative to existing GEVIs. We used Voltron for in vivo voltage imaging in mice, zebrafish, and fruit flies. In the mouse cortex, Voltron allowed single-trial recording of spikes and subthreshold voltage signals from dozens of neurons simultaneously over a 15-minute period of continuous imaging. In larval zebrafish, Voltron enabled the precise correlation of spike timing with behavior.
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