Journal
REPRODUCTIVE TOXICOLOGY
Volume 86, Issue -, Pages 68-75Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.reprotox.2019.02.008
Keywords
Sertoli cells; Oxidative stress; Monobutyl phthalate; Panax ginseng; GIM-1
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Funding
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brasil (CAPES) [001]
- Sao Paulo State Research Foundation (FAPESP) [2012/00253-3]
- National Council for Scientific and Technological Development (CNPq) [306900/2016-5]
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This study evaluated oxidative stress markers in Human Sertoli cells cultivated on Geltrex (R) and exposed to Monobutyl Phthalate (MBP), and the potential cytoprotective role of GIM-1 on the antioxidant response. Exposure was performed at 30 min, 1, 12 and 48 h into 4 groups: control, MBP (10 mu M), GIM-1 (0,05 mu M) and MBP + GIM-1. Morphology was evaluated. Antioxidant enzymes were analyzed by colorimetric method; NRF-2, SIRT-1, 8- OHdG and Cleaved Caspase-3 by Western Blot. Larger spaces between cells were shown in MBP treatment; GIM-1 was similar to Control and MBP + GIM-1 showed an intermediate aspect. MBP reduced enzymatic activity of all enzymes and NRF-2 expression, increasing cleaved Caspase-3 expression; while GIM-1 increased antioxidants markers alone and attenuated MPB effects in MBP + GIM-1. MBP induced deleterious effects on Sertoli cells, increasing the oxidative stress, apoptosis and modifying their distribution in culture; however, GIM-1 acted as an important cytoprotective agent reversing our attenuating MBP effects.
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