Journal
PROTEIN SCIENCE
Volume 28, Issue 8, Pages 1412-1422Publisher
WILEY
DOI: 10.1002/pro.3668
Keywords
Escherichia coli; E; coli strains; recombinant protein production; expression plasmid; affinity tags; fusion partners; inclusion bodies
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Funding
- Fondo para la Investigacion Cientifica y Tecnologica [PICT2015-2955]
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The production of proteins in sufficient amounts is key for their study or use as biotherapeutic agents. Escherichia coli is the host of choice for recombinant protein production given its fast growth, easy manipulation, and cost-effectiveness. As such, its protein production capabilities are continuously being improved. Also, the associated tools (such as plasmids and cultivation conditions) are subject of ongoing research to optimize product yield. In this work, we review the latest advances in recombinant protein production in E. coli.
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