Journal
PROCESS BIOCHEMISTRY
Volume 82, Issue -, Pages 189-198Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2019.04.014
Keywords
Arthrospira; Charcoal; Extraction; Phycobiliprotein; Purity
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Funding
- Centre of Excellence on Agricultural Biotechnology, Science and Technology Postgraduate Education and Research Development Office, Office of the Higher Education Commission, Ministry of Education (AG-BIO/PERDO - CHE) Thailand
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Phycobiliproteins are essential bioactive substances from Arthrospira platensis which have useful biological applications as food additives. Extraction processes of phycobiliproteins and C-phycocyanin purification play significant roles in determining the quality of C-phycocyanin. Oven-dried biomass preparation and concentration by physical-assisted methods were compared to evaluate C-phycocyanin extraction. Although freeze drying was determined as the most suitable drying process, convective drying showed easy disruption and released bioactive compounds as phycobiliproteins. The most suitable processing conditions for extraction and purification of C-PC are oven-dried biomass preparation (at 70 degrees C for 4 h) and extraction (at 25 degrees C for 24 h) by 0.01 M phosphate buffer using homogenisation assisted method at 0.02 g/mL biomass concentration followed by purification using activated charcoal (70-80 g/L for 24 h). At the standardised conditions, the highest purity index of 1.2 with satisfactory yield of around 80% was achieved. Increased purity index also decreased the unfavourable smell of protein in solution.
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