Mechanical Heterogeneity in Tissues Promotes Rigidity and Controls Cellular Invasion

We study the influence of cell-level mechanical heterogeneity in epithelial tissues using a vertex-based model. Heterogeneity is introduced into the cell shape index (p(0)) that tunes the stiffness at a single-cell level. The addition of heterogeneity can always enhance the mechanical rigidity of the epithelial layer by increasing its shear modulus, hence making it more rigid. There is an excellent scaling collapse of our data as a function of a single scaling variable f(r), which accounts for the overall fraction of rigid cells. We identify a universal threshold f(r)* that demarcates fluid versus solid tissues. Furthermore, this rigidity onset is far below the contact percolation threshold of rigid cells. These results give rise to a separation of rigidity and contact percolation processes that leads to distinct types of solid states. We also investigate the influence of heterogeneity on tumor invasion dynamics. There is an overall impedance of invasion as the tissue becomes more rigid. Invasion can also occur in an intermediate heterogeneous solid state that is characterized by significant spatial-temporal intermittency.