4.8 Article

Continuous evolution of base editors with expanded target compatibility and improved activity

Related references

Note: Only part of the references are listed.
Letter Biotechnology & Applied Microbiology

CRISPResso2 provides accurate and rapid genome editing sequence analysis

Kendell Clement et al.

NATURE BIOTECHNOLOGY (2019)

Article Biochemical Research Methods

Phage-Assisted Evolution of Bacillus methanolicus Methanol Dehydrogenase 2

Timothy B. Roth et al.

ACS SYNTHETIC BIOLOGY (2019)

Article Biotechnology & Applied Microbiology

Orthologous CRISPR-Cas9 enzymes for combinatorial genetic screens

Fadi J. Najm et al.

NATURE BIOTECHNOLOGY (2018)

Article Biotechnology & Applied Microbiology

Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction

Luke W. Koblan et al.

NATURE BIOTECHNOLOGY (2018)

Article Biotechnology & Applied Microbiology

Optimized base editors enable efficient editing in cells, organoids and mice

Maria Paz Zafra et al.

NATURE BIOTECHNOLOGY (2018)

Article Multidisciplinary Sciences

A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9

Lun Cui et al.

NATURE COMMUNICATIONS (2018)

Article Multidisciplinary Sciences

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

Johnny H. Hu et al.

NATURE (2018)

Letter Biotechnology & Applied Microbiology

A call for science-based review of the European court's decision on gene-edited crops

Fyodor D. Urnov et al.

NATURE BIOTECHNOLOGY (2018)

Article Biotechnology & Applied Microbiology

Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion

Xiao Wang et al.

NATURE BIOTECHNOLOGY (2018)

Article Biotechnology & Applied Microbiology

An APOBEC3A-Cas9 base editor with minimized bystander and off-target activities

Jason M. Gehrke et al.

NATURE BIOTECHNOLOGY (2018)

Article Biochemistry & Molecular Biology

Continuous directed evolution of proteins with improved soluble expression

Tina Wang et al.

NATURE CHEMICAL BIOLOGY (2018)

Review Genetics & Heredity

Base editing: precision chemistry on the genome and transcriptome of living cells

Holly A. Rees et al.

NATURE REVIEWS GENETICS (2018)

Article Biochemical Research Methods

Comprehensive Profiling of Four Base Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly

Vladimir Potapov et al.

ACS SYNTHETIC BIOLOGY (2018)

Article Multidisciplinary Sciences

Programmable base editing of A.T to G.C in genomic DNA without DNA cleavage

Nicole M. Gaudelli et al.

NATURE (2017)

Article Biotechnology & Applied Microbiology

Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions

Y. Bill Kim et al.

NATURE BIOTECHNOLOGY (2017)

Article Biochemistry & Molecular Biology

Continuous directed evolution of aminoacyl-tRNA synthetases

David Bryson et al.

NATURE CHEMICAL BIOLOGY (2017)

Review Biochemistry & Molecular Biology

Refining strategies to translate genome editing to the clinic

Tatjana I. Cornu et al.

NATURE MEDICINE (2017)

Article Multidisciplinary Sciences

Phage-assisted continuous evolution of proteases with altered substrate specificity

Michael S. Packer et al.

NATURE COMMUNICATIONS (2017)

Article Multidisciplinary Sciences

Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance

Ahmed H. Badran et al.

NATURE (2016)

Article Multidisciplinary Sciences

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

Alexis C. Komor et al.

NATURE (2016)

Article Biochemistry & Molecular Biology

Influence of major-groove chemical modifications of DNA on transcription by bacterial RNA polymerases

Veronika Raindlova et al.

NUCLEIC ACIDS RESEARCH (2016)

Review Biochemistry & Molecular Biology

The APOBEC Protein Family: United by Structure, Divergent in Function

Jason D. Salter et al.

TRENDS IN BIOCHEMICAL SCIENCES (2016)

Review Biochemistry & Molecular Biology

In vivo continuous directed evolution

Ahmed H. Badran et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2015)

Article Multidisciplinary Sciences

Development of potent in vivo mutagenesis plasmids with broad mutational spectra

Ahmed H. Badran et al.

NATURE COMMUNICATIONS (2015)

Article Biochemical Research Methods

Accurate DNA Assembly and Genome Engineering with Optimized Uracil Excision Cloning

Ana Mafalda Cavaleiro et al.

ACS SYNTHETIC BIOLOGY (2015)

Article Biochemical Research Methods

A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly

Michael E. Lee et al.

ACS SYNTHETIC BIOLOGY (2015)

Article Biochemistry & Molecular Biology

IQ-TREE: A Fast and Effective Stochastic Algorithm for Estimating Maximum-Likelihood Phylogenies

Lam-Tung Nguyen et al.

MOLECULAR BIOLOGY AND EVOLUTION (2015)

Article Biotechnology & Applied Microbiology

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

Shengdar Q. Tsai et al.

NATURE BIOTECHNOLOGY (2015)

Article Biochemistry & Molecular Biology

Negative selection and stringency modulation in phage-assisted continuous evolution

Jacob C. Carlson et al.

NATURE CHEMICAL BIOLOGY (2014)

Article Multidisciplinary Sciences

A system for the continuous directed evolution of proteases rapidly reveals drug-resistance mutations

Bryan C. Dickinson et al.

NATURE COMMUNICATIONS (2014)

Review Clinical Neurology

Apolipoprotein E and Alzheimer disease: risk, mechanisms and therapy

Chia-Chan Liu et al.

NATURE REVIEWS NEUROLOGY (2013)

Article Multidisciplinary Sciences

Experimental interrogation of the path dependence and stochasticity of protein evolution using phage-assisted continuous evolution

Bryan C. Dickinson et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)

Article Pathology

Identification of Three Novel Hearing Loss Mouse Strains with Mutations in the Tmc1 Gene

Shehnaaz S. M. Manji et al.

AMERICAN JOURNAL OF PATHOLOGY (2012)

Article Biochemistry & Molecular Biology

FastML: a web server for probabilistic reconstruction of ancestral sequences

Haim Ashkenazy et al.

NUCLEIC ACIDS RESEARCH (2012)

Article Multidisciplinary Sciences

A system for the continuous directed evolution of biomolecules

Kevin M. Esvelt et al.

NATURE (2011)

Article Biochemistry & Molecular Biology

Design, construction and characterization of a set of insulated bacterial promoters

Joseph H. Davis et al.

NUCLEIC ACIDS RESEARCH (2011)

Article Biochemical Research Methods

I-TASSER: a unified platform for automated protein structure and function prediction

Ambrish Roy et al.

NATURE PROTOCOLS (2010)

Article Biochemistry & Molecular Biology

A Portable Hot Spot Recognition Loop Transfers Sequence Preferences from APOBEC Family Members to Activation-induced Cytidine Deaminase

Rahul M. Kohli et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2009)

Article Multidisciplinary Sciences

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

Carola Engler et al.

PLOS ONE (2008)

Article Biochemistry & Molecular Biology

T-Coffee: A novel method for fast and accurate multiple sequence alignment

C Notredame et al.

JOURNAL OF MOLECULAR BIOLOGY (2000)