Journal
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 19, Issue -, Pages 95-105Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nano.2019.03.013
Keywords
miRNA; RNA delivery; Nanoparticles; Mammary gland; Stem cells; Breast cancer
Funding
- Associazione Italiana per la Ricerca sul Cancro [AIRC-G14085, IG18774]
- Cariplo [2015-0590]
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Functional RNAs, such as microRNAs, are emerging as innovative tools in the treatment of aggressive and incurable cancers. In this study, we explore the potential of silica dioxide nanoparticles (SiO(2)NPs) in the delivery of biologically active miRNAs. Focusing on the tumor-suppressor miR-34a, we evaluated miRNAs delivery by SiO(2)NPs into the mammary gland, using in vitro as well as in vivo model systems. We showed that silica nanoparticles can efficiently deliver miR-34a into normal and cancer epithelial cells grown in culture without major signs of toxicity. Delivered miRNA retained the ability to silence artificial as well endogenous targets and can reduce the growth of mammospheres in 3D culture. Finally, miR-34a delivery through intra-tumor administration of SiO(2)NPs leads to a reduced mammary tumor growth. In conclusion, our studies suggest that silica nanoparticles can mediate the delivery of miR-34a directly into mammary tumors while preserving its molecular and biological activity. (C) 2019 The Author(s). Published by Elsevier Inc.
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