Journal
NANOMEDICINE
Volume 14, Issue 11, Pages 1387-1401Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/nnm-2018-0462
Keywords
c-MYC; cancer; gene silencing; gold nanoparticles; poly(ethylene glycol); siRNA
Funding
- National Research Foundation of South Africa [113850]
Ask authors/readers for more resources
Aim: To produce sterically stabilized and functionalized gold nanoparticles (AuNPs) for efficient delivery of siRNA for c-MYC silencing in vitro. Materials & methods: Synthesized AuNPs were functionalized with chitosan and PEG(400) and PEG(2000), morphologically and chemically characterized, and assessed for cytotoxicity and gene silencing in vitro. Results & discussion: AuNPs presented as spherical particles in the nanometer size range and successfully bound and protected the siRNA against degradation and were well tolerated in the breast adenocarcinoma (MCF-7) cell line. Nanoparticle-mediated gene knockdown studies revealed enhanced levels of c-MYC gene silencing with more than 90% reduction of MYC protein levels. Conclusion: These nanoformulations show enhanced potential for siRNA-mediated gene silencing in human breast cancer cells in vitro.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available