Journal
MOLECULAR NUTRITION & FOOD RESEARCH
Volume 63, Issue 19, Pages -Publisher
WILEY
DOI: 10.1002/mnfr.201801390
Keywords
dendritic cells; inflammation; inflammatory bowel disease; microRNAs; quercetin
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Funding
- Italian Ministry of Health [GR-2011-02350438, 13/2018]
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Scope Dendritic cells (DCs) are the most potent antigen-presenting cells that play an important role in the crosstalk between the innate and the adaptive immune response. Quercetin exposure is identified as an effective strategy to suppress the inflammatory response induced by LPS. Methods and results In this study, using a next-generation sequencing analysis, the effect of quercetin on microRNAs (miRNAs) expression in DCs is examined. A signature of 113 miRNAs that are differentially regulated in LPS-stimulated DCs after quercetin exposure is defined. It is demonstrated that the loss of function of miR-369-3p in LPS-stimulated DCs during quercetin exposure led to an increase of CCAAT/enhancer binding protein beta (C/EBP-beta) mRNA and protein and its downstream targets tumor necrosis factor-alpha (TNF-alpha) and interleukin 6 (IL6). Conversely, it is shown that the ectopic induction of miR-369-3p without quercetin suppresses the inflammatory response of LPS reducing C/EBP-beta, TNF-alpha, and IL6 production. In vivo, oral administration of quercetin in dextran-sulfate-sodium-induced colitis induces miR-369-3p expression. Conclusions These findings indicate that quercetin-induced miR-369-3p regulates the inflammatory cascade in chronic inflammatory response and present promising therapeutic implications.
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