Journal
MICROCHIMICA ACTA
Volume 186, Issue 8, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-019-3615-2
Keywords
Heavy metals; DNA nanotechnology; MNAzyme; DNA probe; Molecular computing; Building materials; Catalytic nucleic acids; Three-way junction; Nonenzymatic system
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Funding
- Wanzhou District Science and Technology Project [wzstc-2018008]
- Ph.D. Programs Foundation of Chongqing Three Gorges Central Hospital [2017BSKYQDJJ06]
- National Natural Science Foundation of China [81860380]
- Fund of Guizhou Provincial Chinese Medicine Administration [QZYY-2018-084]
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A nonenzymatic fluorometric assay is described for highly sensitive and selective detection of silver ion. It is making use of a controlled DNA assembly and an AND logic operation of a multiple-component DNAzyme (MNAzyme). It corresponds to an Ag(I)-responsive three-way junction (3-WJ) assembly. The tailored probes of the 3-WJ architecture were designed with complementary domains for subsequent assembly. Cytosine (C)-cytosine mismatches at one-way junction were set as the sensing element for Ag(I). Upon exposure to Ag(I) as an input, C-Ag(I)-C pairs are being formed. This enhances the binding energy between these separate probes and thus promotes the formation of a nanostructure that represents an AND logic assembly of MNAzyme with an amplified output signal. This results in an Ag(I)-induced increase in fluorescence which is measured best at excitation/ emission maxima of 645/ 670 nm. The method displays high selectivity and sensitivity, has a 5 pM detection limit at 3s and a dynamic range that extends from 10 pM to 100 nM.
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