Journal
MICROCHIMICA ACTA
Volume 186, Issue 7, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-019-3569-4
Keywords
Carbon nanoparticles; Nanoprobes; m-Phenylenediamine; Assistance of H2SO4; Fluorescence; Quantum yield; Excellent photostability; Intracellular pH change; Bioimaging; Real-time sensing
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Funding
- National Natural Science Foundation of China [21807068, 21373132, 21502109]
- Natural Science Foundation of Shaanxi Province [2017JQ2017]
- Project of Shaanxi University of Technology [SLGKY14-08]
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New green-emissive carbon dots (G-CDs) are described here and shown to be viable fluorescent nanoprobes for the detection of changes in cellular pH values. By using m-phenylenediamine as the carbon source, G-CDs with an absolute quantum yield of 36% were solvothermally synthesized in the presence of strong H2SO4. The G-CDs have an average size of 2.3nm and display strong fluorescence with excitation/emission peaks at 450/510nm. The fluorescence intensity depends on the pH value in the range from 6.0 to 10.0, affording the capability for sensitive detection of intracellular pH variation. The nanosensor with excellent photostability exhibited good fluorescence reversibility in different pH solutions, and showed excellent stability against the influence of other biological species. The nanoprobe was successfully used in confocal fluorescence microscopy to determine pH values in SMMC-7721 cells.
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