Journal
METABOLIC ENGINEERING
Volume 54, Issue -, Pages 83-95Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2019.03.003
Keywords
Yarrowia lipolytica; Ionic liquid; Adaptive laboratory evolution; Stress responsive metabolism; Lipidomics; Metabolomics; Glycerophospholipids; Sterols; Sterol transcription factor
Categories
Funding
- BioEnergy Science Center (BESC)
- Center for Bioenergy Innovation (CBI)
- U.S. Department of Energy (DOE) Bioenergy Research Centers - Office of Biological and Environmental Research in the DOE Office of Science (DOE BER)
- Office of Science of the U.S. DOE [DE-AC02-05CH11231]
- National Science Foundation (NSF) [1511881]
- DOE BER Genomic Science Program [DE-SC0019412]
- U.S. Department of Energy (DOE) [DE-SC0019412] Funding Source: U.S. Department of Energy (DOE)
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Green organic solvents such as ionic liquids (ILs) have versatile use but are inhibitory to microbes even at low concentrations of 0.5-1.0% (v/v) ILs. We discovered the oleaginous yeast Yarrowia lipolytica can grow in 10% (v/v) of 1-ethyl-3-methylimidazolium acetate ([EMIM] [OAc]), which makes it more tolerant than most engineered microorganisms and naturally screened isolates. However, the underlying mechanism of IL tolerance in Y. lipolytica is not understood. Through adaptive laboratory evolution, in combination with physiological characterization and omics analysis, we shed light on the underlying mechanism of how Y. lipolytica restructures its membrane to tolerate different types of ILs at high levels up to 18% ILs. Specifically, we discovered that sterols play a key role for exceptional IL tolerance in Y. lipolytica.
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