Journal
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 99, Issue -, Pages 1502-1508Publisher
ELSEVIER
DOI: 10.1016/j.msec.2019.02.065
Keywords
Screen-printed electrodes; Capacitive biosensors; Low-cost sensors; Carbon nano-onions; CA19-9; information visualization
Categories
Funding
- CAPES [001]
- CNPq
- INEO
- FAPESP [2012/15543-7, 2013/14262-7, 2015/01770-0, 2016/00991-5, 2017/12096-3]
- Vicerrectoria de Investigaciones
- Centro de Excelencia en Nuevos Materiales (CENM) from the Universidad del Valle
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [12/15543-7] Funding Source: FAPESP
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Nanostructured capacitive biosensors, combined with inexpensive fabrication technologies, may provide simple, sensitive devices for detecting clinically relevant cancer biomarkers. Herein, we report a novel platform for detecting the pancreatic cancer biomarker CA19-9 using low-cost screen-printed interdigitated electrodes (SPIDEs). The SPIDEs were modified by carbon nano-onions (CNOs) and graphene oxide (GO) films, on which a layer of anti-CA19-9 antibodies was immobilized. The modification with CNOs and GO significantly improved the analytical performance of the biosensor, which displayed superior results to those prepared only with GO. The biossensor exhibited high reproducibility and a relatively low limit of detection of 0.12 U mL(-1). Using these devices in combination with information visualization methods we were able to detect CA19-9 in whole cell lysates of colorectal adenocarcinoma. The fabrication of these low-cost, disposable inummosensors is a successful attempt to explore CNOs in capacitive biosensors, which may be extended for detection of different cancer biomarkers.
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