Flurbiprofen loaded ethosomes - transdermal delivery of anti-inflammatory effect in rat model

BackgroundEthosomes have been widely used in Transdermal Drug Delivery System (TDDS) as they increase the permeation of drug across the skin.MethodsFlurbiprofen loaded vesicular ethosomes were formulated, optimized and characterized for particle size, entrapment efficiency, poly dispersive index (PDI), microscopy using Atomic force microscopy (AFM), Scanning electron microscope (SEM) and Transmission electron microscopy (TEM) and Interaction of drug and excipients were studied using Fourier transform infra-red (FTIR) spectroscopy, Differential scanning colorimetry (DSC), Thermo gravimetric analysis (TGA). Further, ethosomal formulations of flurbiprofen were evaluated for stability study of three months and in vitro drug permeation study was carried out using albino rat skin. In addition, skin irritation test was evaluated by Draize test and in vivo study of prepared formulation was examined through paw edema assay by inducing carrageenan and cold plate method.ResultsAmongst all formulations, EF5 formulation exhibited ideal surface morphology, with maximum entrapment efficiency (95%) with optimal excipient concentration i.e. 200mg phospholipid and 35% ethanol. The ideal vesicle size was achieved as 162.22nm, with zeta potential -48.14 +/- 1.4mV with the PDI of 0.341. In-vitro permeation study shows a release of 82.56 +/- 2.11g/cm(2) in 24h and transdermal flux was found as 226.1g/cm(2)/h. Cold plate test indicates that the formulation EF5 showed a marked analgesic activity and Carrageenan induced paw edema test indicates that the formulation EF5 inhibits the increase in paw edema. Ethosomal suspension at 4 degrees C showed maximum stability.Conclusions The overall study concluded that this ethosomal approach offers a new delivery system for sustained and targeted delivery for flurbiprofen.