Journal
JOURNAL OF TRANSLATIONAL MEDICINE
Volume 17, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12967-019-1954-8
Keywords
Colorectal cancer; Biomarkers; Diagnostic; miRNAs; Ascitic fluid; Peritoneal lavage; Liquid biopsy; Extracellular vesicles; Colon cancer
Categories
Funding
- Departament de Salut of the Generalitat de Catalunya [SLT002/16/00274]
- CIBERONC [CB16/12/00231, CB16/12/00328]
- Grups consolidats de la Generalitat de Catalunya [2017SGR1368, 2017SGR1661]
- Plataforma biobancos [PT17/0015/0027]
- IRBLleida BIOBANK [B.0000682]
- [ISCIII PIE15/00029]
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BackgroundColorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk.MethodsIn this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fluids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 371 miRNAs.Results210 miRNAs were significantly dysregulated (adjusted p value<0.05 and abs(logFC)1). The top-10 miRNAs, which had the AUC value higher than 0.95, were miRNA-199b-5p, miRNA-150-5p, miRNA-29c-5p, miRNA-218-5p, miRNA-99a-3p, miRNA-383-5p, miRNA-199a-3p, miRNA-193a-5p, miRNA-10b-5p and miRNA-181c-5p.ConclusionsThis finding opens the avenue to the use of EV-associated miRNA of peritoneal lavages as an untapped source of biomarkers for CRC.
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