Journal
APPLIED SURFACE SCIENCE
Volume 360, Issue -, Pages 28-36Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.apsusc.2015.10.178
Keywords
Amine coating; Sensors; Bio-immobilization; Plasma polymerization; Cyclopropylamine; XPS; QCM
Categories
Funding
- BioFibPlas project [35GA5652]
- SoMoPro II Programme
- People Programme (Marie Curie Action) of the Seventh Framework Programme of EU [291782]
- South-Moravian Region
- projects CEITEC - Central European Institute of Technology [CZ.1.05/1.1.00/02.0068]
- European Regional Development Fund
- Seventh Framework Programme of EU
- project Materials Research Centre at FCH BUT - Sustainability and Development [REG L01211]
- Structural Analysis Laboratory of CEITEC - Central European Institute of Technology under CEITEC - open access project [LM2011020]
- Ministry of Education, Youth and Sports of the Czech Republic [IZ73Z0_152661]
- Swiss National Science Foundation
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The performance of immunosensing devices crucially depends on the methodology of antibody or antigen immobilization on the sensor surface. Hence, the stable intermediate layers capable of specific and reproducible binding of antibodies are required. Herein, we introduce the amine rich (NHx concentration of 6 at.%) layers prepared by pulsed plasma polymerization of cyclopropylamine (CPA) for functionalization of the quartz crystal microbalance (QCM) surface by the antibody specific to human serum albumin. In these layers the amine groups serve as anchor for the antibody binding. The sensitivity of QCM sensors prepared in this way surpasses the one for the previously reported sensors functionalized by the thiolbased self-assembled monolayers by the factor of 2. Our results thus show that CPA plasma polymers have a significant potential for further development of the active layers for biosensing applications. (C) 2015 Elsevier B.V. All rights reserved.
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