Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
Volume 380, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotochem.2019.05.005
Keywords
Nanohybrids; Photodynamic therapy (PDT); Squaraine (SQ); Zinc oxide nanoparticles (ZnO NPs); Forster resonance energy transfer (FRET); Photo-induced charge transfer; Drug aggregation
Categories
Funding
- Department of Science and Technology (DST, India)
- DST-SERB [EMR/2016/004698, DBT-BT/PR11534/NNT/28/766/2014]
Ask authors/readers for more resources
Photodynamic therapy (PDT) is a clinically approved, minimally invasive therapeutic procedure exhibiting cytotoxic effects toward malignant cells. Hydrophobic nature of most photosensitizers used in PDT with lower light absorption ability restricts practical use of PDT. Herein, we have employed a squaraine drug (SQ) with 665 nm absorbance peak maxima as the photosensitizing agent and evaluate its photo-physical properties. The tendency of aggregation formation in aqueous media limits its practical usefulness. Thus, we have synthesised wide band gap semiconductor zinc oxide (ZnO) nanoparticles and functionalized the surface using squaraine molecules. The molecular cross-talking was evaluated using excited state fluorescence lifetime decay profiles and by employing Forster resonance energy transfer (FRET) technique. The nanohybrids show improvement in three aspects compared to bare SQ molecule such as lesser aggregate formation in aqueous media, pH responsive precipitation of the drug and improvement of photo-induced reactive oxygen species (ROS) generation. Ultrafast dynamical study at the inorganic (ZnO) - organic (SQ) interface depicts presence of photo-induced charge transfer process at the junction which indeed improves the ROS generation capability. Finally, the photodynamic action has been evaluated in human breast cancer cell line MCF-7. The nanohybrids depict enhanced photo toxicity in cancer cell with loss of adherence and typical morphology of cancerous cell depicting controlled cell death. The present study employ characterisation of nanohybrids for potential use in PDT for cancer treatment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available