Journal
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
Volume 69, Issue 2, Pages 235-238Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/MPG.0000000000002363
Keywords
casein; fat globules; protocols; stability
Funding
- National Institute of Food and Agriculture (NIFA), U.S. Department of Agriculture [2015-67017-23181, NIFA2016-67001-25301]
- National Institutes of Health (NIH) [1P20GM104320]
- U.S. Department of Agriculture multistate group [W3002]
- University of Nebraska President's Office
- University of Nebraska Agricultural Research Division (Hatch Act)
Ask authors/readers for more resources
The objectives of this study were to lay the methodological groundwork for field studies of microRNA analysis in exosomes from small sample volumes of human milk, and assess exosome and microRNA content in infant formulas. When human milk was stored at 4 degrees C for 4 weeks, the count of exosome-sized vesicles decreased progressively to 49% +/- 13% of that in fresh milk. Exosomes were purified from 1 mL of fresh human milk and their microRNA content was assessed by microRNA-sequencing analysis and compared with that in infant formulas. We identified 221 microRNAs in exosomes from 3 samples of fresh human milk; 84 microRNAs were present in all 3 samples. MicroRNAs were not detectable in infant formulas and their exosome-sized vesicles, which appeared to be casein micelles. We conclude that large-scale studies of microRNAs in human milk exosomes are feasible, and exosomes and microRNAs are not detectable in formulas.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available