Journal
BIOMEDICAL PAPERS-OLOMOUC
Volume 159, Issue 1, Pages 12-17Publisher
PALACKY UNIV, MEDICAL FAC
DOI: 10.5507/bp.2013.076
Keywords
chemical decellularization; detergent; extracellular matrix; cell lysis
Funding
- Internal Competition of Medical Faculty in Hradec Kralove [PRVOUK P37/06]
- Charles University in Prague [SVV-2012-264901, 736213]
- European Social Fund
- state budget of the Czech Republic [CZ.1.07/2.3.00/30.0022]
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Background. A biological scaffold from extracellular matrix can be produced by a variety of decellularization methods whose caveat consists in efficiently eliminating cells from the treated tissue. This scaffold can be used in diverse applications for tissue engineering and organ regeneration. Preservation of the extracellular matrix ultrastructure is highly desirable because of its unique architecture, contained growth factors and decreased immunological response. All of these properties provide attachment sites and adequate environment for cells colonizing this scaffold, reconstituting the decellularized organ. This review briefly describes chemical decellularization methods, evaluation of these protocols and the role of ECM in tissue engineering. Conclusion. Chemical decellularization is an often used method for scaffold preparation and makes possible a well-preserved three dimensional structure of extracellular matrix.
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