Journal
JOURNAL OF INNATE IMMUNITY
Volume 12, Issue 2, Pages 170-181Publisher
KARGER
DOI: 10.1159/000499841
Keywords
Neutrophils; Nonhuman primate; Phagocytosis; AIDS; Antiretroviral treatment; Mass cytometry
Categories
Funding
- Programme Investissements d'Avenir (PIA) [ANR11-INBS-0008, ANR-10-EQPX-02-01]
- ANRS
- MSD Avenir, pVISCONTI research program
- SIVART ANRS-IDMIT CO1 research program
Ask authors/readers for more resources
The infected host fails to eradicate HIV-1, despite significant control of viral replication by combinational antiretroviral therapy. Here, we assessed the impact of HIV infection on immune-cell compartments in a SIVmac251 nonhuman primate infection model, which allowed the choice of contamination route, time of infection, and treatment follow-up. We performed high-throughput multiparameter single-cell phenotyping by mass cytometry to obtain a global vision of the immune system in blood and bone marrow. Circulating polymorphonuclear neutrophils (PMNs) with impaired phagocytosis had altered surface expression of CD62L and CD11b during early chronic infection. The initiation of combinational antiretroviral treatment during primary infection did not restore PMN function. The maturation state of PMNs was highly altered during late chronic SIV infection, showing a primarily immature phenotype. Our results provide new insights into PMN involvement in the pathogenesis of HIV infection and may play a role in the establishment and maintenance of chronic immune activation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available