Fluorescence Off-On Probe for L-Cysteine Detection Based on Nitrogen Doped Carbon Dots

Herein, a simple and efficient fluorescence analysis method for L-Cysteine (L-Cys) was established. The method was based on the fluorescent off-on mode of nitrogen doped carbon dots (NCDs). The NCDs were prepared via a facile one-step solvothermal method. In the process of exploring the bio-functional application of these newly synthesized NCDs, we found these NCDs with rich functional groups exhibited excellent optical properties. In addition, these newly synthesized NCDs showed an excitation-dependent emissions photolumine-scent (PL) property and exhibited good performance in the detection of Fe3+ ions by quenching the blue emission fluorescence. Interestingly, the quenched fluorescence of NCDs was recovered with the addition of L-Cys, which provided a novel approach for L-Cys detection. The NCDs-based fluorescent off-on sensor has a wide linear detection range (0-100 mu M), and a relatively low detection limits (0.35 mu M) for L-Cys. This simple fluorescent off-on approach is, very sensitive and selective for L-Cys detection, which also provides a new insight on NCDs biosensor application.