Resveratrol anchored nanostructured lipid carrier loaded in situ gel via nasal route: Formulation, optimization and in vivo characterization

The purpose of the present study was to formulate resveratrol loaded nanostructured lipid carrier based in situ gel for intranasal administration. Resveratrol loaded lipid carrier was prepared by a melt emulsification-probe sonication method, characterized and optimized. Nanostructured lipid carrier was then incorporated into in situ gel and characterized. The resveratrol nanostructured lipid carrier was formulated and evaluated for particle size (132 +/- 12 nm), polydispersity index (0.165 +/- 0.002), zeta potential (-23 +/- 4 mV), drug loading (10 +/- 3%) and entrapment efficiency (74 +/- 6%). A pharmacokinetic study showed the higher drug distribution in the brain from in situ gel which indicated safety and efficacy of developed formulation upon administration through nasal route. Thus, we can conclude that partially drug might have absorbed via an olfactory route from developed formulation and hence the intranasal route could be a promising approach for the treatment of Alzheimer's disease.