Journal
JOURNAL OF CELLULAR PHYSIOLOGY
Volume 234, Issue 12, Pages 21838-21850Publisher
WILEY
DOI: 10.1002/jcp.28660
Keywords
adipose tissue; differentiation; microRNAs; pigs; proliferation
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Funding
- Major Projects for Genetically Modified Organisms Breeding [2014ZX08009-047B]
- National Natural Science Foundation of China [31572366]
- National Key Basic Research and Development Program [2015CB943102]
- National Science and Technology Major Project of China [2016ZX08006003]
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MicroRNAs (miRNAs) have critical roles during adipogenesis; however, their precise functions are not completely understood. Porcine miRNA expression profiles show that miR-127 is dramatically downregulated with age in adipose tissue. We aimed to identify the precise functions and mechanisms of miR-127 in proliferation and adipogenesis. Preadipocytes were cultured under conditions to induce proliferation or differentiation and the effect of miR-127 overexpression on these processes, and the associated bioinformatically predicted target genes, were assessed using luciferase assays, quantitative real-time PCR, western blot analysis, and cell staining techniques. miR-127 increased proliferation by promoting cell cycling, whereas it suppressed differentiation, which was accompanied by reduced lipid accumulation. miR-127 targeted mitogen-activated protein kinase 4 and homeobox C6 (HOXC6) to activate preadipocyte proliferation. During differentiation, miR-127 targeted HOXC6 to attenuate adipogenesis. These findings identify miR-127 as an inhibitor of porcine adipogenesis, which may inform future strategies to reduce porcine fat deposition and treat human obesity.
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