Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 294, Issue 35, Pages 13006-13016Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.RA119.007730
Keywords
plant defense; protein structure; plant biochemistry; host-pathogen interaction; Nod-like receptor (NLR); effector; plant immunity; rice; rice blast disease
Categories
Funding
- Biotechnology and Biological Sciences Research Council (United Kingdom) [BB/P012574, BB/M02198X]
- European Research Council [743165]
- John Innes Foundation
- Gatsby Charitable Foundation
- JSPS KAKENHI [15H05779, 18K05657]
- BBSRC [BB/M022315/1, BBS/E/J/000PR9798, BB/M02198X/1, BBS/E/J/000PR9795] Funding Source: UKRI
- Grants-in-Aid for Scientific Research [15H05779, 18K05657] Funding Source: KAKEN
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Unconventional integrated domains in plant intracellular immune receptors of the nucleotide-binding leucine-rich repeat (NLRs) type can directly bind translocated effector proteins from pathogens and thereby initiate an immune response. The rice (Oryza sativa) immune receptor pairs Pik-1/Pik-2 and RGA5/RGA4 both use integrated heavy metal-associated (HMA) domains to bind the effectors AVR-Pik and AVR-Pia, respectively, from the rice blast fungal pathogen Magnaporthe oryzae. These effectors both belong to the MAX effector family and share a core structural fold, despite being divergent in sequence. How integrated domains in NLRs maintain specificity of effector recognition, even of structurally similar effectors, has implications for understanding plant immune receptor evolution and function. Here, using plant cell death and pathogenicity assays and protein-protein interaction analyses, we show that the rice NLR pair Pikp-1/Pikp-2 triggers an immune response leading to partial disease resistance toward the mis-matched effector AVR-Pia in planta and that the Pikp-HMA domain binds AVR-Pia in vitro. We observed that the HMA domain from another Pik-1 allele, Pikm, cannot bind AVR-Pia, and it does not trigger a plant response. The crystal structure of Pikp-HMA bound to AVR-Pia at 1.9 angstrom resolution revealed a binding interface different from those formed with AVR-Pik effectors, suggesting plasticity in integrated domain-effector interactions. The results of our work indicate that a single NLR immune receptor can bait multiple pathogen effectors via an integrated domain, insights that may enable engineering plant immune receptors with extended disease resistance profiles.
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