Erythrobacter marisflavi sp. nov., isolated from isolated from estuary water

A Gram-stain-negative, aerobic, non-motile and coccoid-, ovoid- or rod-shaped bacterial strain, designated KEM-5(T), was isolated from water sampled at an estuary environment on the Yellow Sea, Republic of Korea. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain KEM-5(T) fell within the clade comprising the type strains of Erythrobacter species. Strain KEM-5(T) exhibited 16S rRNA gene sequence similarities of 97.01-97.66% to the type strains of E. rythrobacter citreus, E. rythrobacter seohaensis and E. rythrobacter pelagi and of 94.18-96.95% to the type strains of the other Erythrobacter species. The genomicaverage nucleotide identity values of strain KEM-5(T) with a non-type strain (LAMA 915) of E. citreus and E. seohaensis SW-135(T) were 76.04 and 74.98 %, respectively. Mean DNA-DNA relatedness values between strain KEM-5(T) and the type strains of E. citreus, E. seohaensis and E. pelagi were 10-18 %. Strain KEM-5(T) contained Q-10 as the predominant ubiquinone and C-18:1 omega 7c, summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), C-16:0 and C-17:1 omega 6c as the major fatty acids. The major polar lipids of strain KEM-5(T) were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. The DNA G+C content of strain KEM-5(T) was 62.4 mol%. Distinguishing phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain KEM-5(T) is separated from recognized Erythrobacter species. On the basis of the data presented here, strain KEM-5(T) is considered to represent a novel species of the genus Erythrobacter, for which the name Erythrobacter marisflavi sp. nov. is proposed. The type strain is KEM-5(T) (=KACC 19865(T)=KCTC 62896(T)=NBRC 113546).