Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 20, Issue 14, Pages -Publisher
MDPI
DOI: 10.3390/ijms20143571
Keywords
comparative genomic hybridization; centric fusion; multiple sex chromosomes; Oplegnathus; whole chromosome painting
Funding
- National Natural Science Foundation of China [31672672]
- Project of Zhejiang Province of China [2017C04003]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [401962/2016-4, 302449/2018-3]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2018/22033-1]
- CAPES/Alexander von Humboldt [88881.136128/2017-01]
- [RVO: 67985904]
- [PPLZ: L200451751]
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Oplegnathus fasciatus and 0. punctatus (Teleostei: Centrarchiformes: Oplegnathidae), are commercially important rocky reef fishes, endemic to East Asia. Both species present an X1X2Y sex chromosome system. Here, we investigated the evolutionary forces behind the origin and differentiation of these sex chromosomes, with the aim to elucidate whether they had a single or convergent origin. To achieve this, conventional and molecular cytogenetic protocols, involving the mapping of repetitive DNA markers, comparative genomic hybridization (CGH), and whole chromosome painting (WCP) were applied. Both species presented similar 2n, karyotype structure and hybridization patterns of repetitive DNA classes. 5S rDNA loci, besides being placed on the autosomal pair 22, resided in the terminal region of the long arms of both X1 chromosomes in females, and on the X1 and Y chromosomes in males. Furthermore, WCP experiments with a probe derived from the Y chromosome of O. fasciatus (OFAS-Y) entirely painted the X1 and X2 chromosomes in females and the X1, X2, and Y chromosomes in males of both species. CGH failed to reveal any sign of sequence differentiation on the Y chromosome in both species, thereby suggesting the shared early stage of neo-Y chromosome differentiation. Altogether, the present findings confirmed the origin of the X1X2Y sex chromosomes via Y-autosome centric fusion and strongly suggested their common origin.
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