Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 130, Issue -, Pages 745-754Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2019.03.023
Keywords
Lentinus edodes polysaccharides; Immunoenhancing effect; MAPK signaling pathway; Submerged culture; Structural properties
Funding
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University
- Fundamental Research Funds for the Central Universities of China [2662015PX199]
- China Scholarship Council [201706765055]
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A crude polysaccharide fraction (termed cLEP) and two derived fractions (termed LEP1 and LEP2) from Lentinus edodes mycelia were purified and characterized. LEP1/-2 were classified as alpha-type heteropolysaccharides with 1 -> 2, 1 -> 3, 1 -> 4 , 1 -> 6 linkages. Their monosaccharide components were respectively Gal, Glc, Man, Ara, Fuc, and Rha (molar ratio 10.17:9.75:9.01:1.61:1.26:1), and Glc, Man, Fuc, Rha, and Gal (molar ratio 5.18:4.69:2.85:1.43:1). In vitro culture experiments with macrophage RAW264.7 cells showed no cytotoxic effects of the polysaccharides. Phagocytosis (neutral red uptake) was significantly enhanced by LEP1/-2. Levels of NO, TNF-alpha and IL-6 were higher in LEP1/-2-treated groups than in cLEP-treated group. qRT-PCR analysis showed that LEP1/-2 had greater enhancing effect on mRNA transcription of iNOS, TNF-alpha, and IL-6 genes. Western blotting analysis revealed that LEP1/-2 strongly promoted phosphorylation of kinases ERK and JNK, and suggested that they exert immunoenhancing effects via MAPK signaling pathway. (C) 2019 Elsevier B.V. All rights reserved.
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