Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 131, Issue -, Pages 420-434Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2019.02.169
Keywords
Chitosan films; Turmeric essential oil; Aspergillus flavus
Funding
- National Natural Science Foundation of China [81603288]
Ask authors/readers for more resources
Here, we studied the preparation, characterization, anti-aflatoxigenic activity, and molecular mechanism in vitro of chitosan packaging films containing turmeric essential oil (TEO). First, we took the mechanical properties as the evaluation Index, screened for the optimum preparation conditions of packaging films with 1.5 mu L/cm(2) TEO using single factor and orthogonal experiments, and characterized the film properties. We found that the addition of TEO affected the microcosmic structure of films and advanced water resistance capacity. In addition, we investigated the inhibitory effects of pure chitosan films and packaging films containing 1.5 mu L/cm(2) or 3.0 mu L/cm(2) TEO on the growth and conidial formation of Aspergillus flavus (A. flavus, CGMCC 3.4410), as well as the accumulation of aflatoxin over the course of seven days. We found that the packaging films possessed a prominent antifungal activity on A. flavus. Finally, we discuss preliminary results surrounding gene expression of packaging films which inhibit aflatoxin biosynthesis. The expressions levels of 16 genes related to afiatoxin biosynthesis were found to be either completely or almost completely inhibited. Therefore, the addition of the natural antifungal agent TEO in chitosan packaging films represent a remarkable method to significantly promote the development and application of antifungal packaging materials. (C) 2019 Published by Elsevier B.V.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available