Antimicrobial, anticoagulant and antiplatelet activities of green synthesized silver nanoparticles using Selaginella (Sanjeevini) plant extract

The recent study deals with the synthesis and characterization of silver nanoparticles bounded to Selaginella bryopteris (Sanjeevini) Plant extract (SPE) as reducing and capping agents. Selaginella bryopteris plant extract silver nanoparticles (SPE@Ag-NPs) was characterized by PXRD, UV-Vis., FESEM, TEM and EDX. The highly stable SPE@Ag-NPs was found promising as antibacterial and antifungal agents when tested against human pathogens S. aureus, E. coli, and A. niger respectively. Furthermore, SPE@Ag-NPs found to show anticoagulant property by enhancing the clotting time of citrated human PRP as well as PPP from the control 160 s to 220 s and 160 s to 284 s respectively. However, SPE@Ag-NPs exhibited mild antiplatelet activity by inhibiting specifically agonist ADP induced platelet aggregation of about 23% at the concentration of 30 mu g. Fascinating, SPE@Ag-NPs did not alter the agonist epinephrine-induced platelet function even at the increased dose. Interestingly, SPE@Ag-NPs did not induce edema and hemorrhage in the experimental mice and also did not hydrolyze RBC cells suggesting its nontoxic property. In conclusion, SPE@Ag-NPs was non-toxic and found to exhibit antibacterial, antifungal, anticoagulant and antiplatelet properties. Thus, SPE@Ag-NPs would be a better candidate in the biomedical field especially to treat thrombotic disorders. (C) 2019 Published by Elsevier B.V.